Read and understand all information in the operating manual. Spark 20m reads all standard ansislas microplate formats up to 1,536 wells. Our integrated and expandable solutions are targeted towards specific user needs with open software and verified protocols for the tecan immunoassay portfolio and are built to ensure compliance with the ivd 9879ec directive. This is an official page of the university of massachusetts medical school.
Before operating the instrument, the user must read and understand this manual. The spark is a multimode plate reader for fluorescence, absorbance, and luminescence measurements. Please read this information before using your phone. Tecan offers microplate readers with both multimode or absorbance reading options. We are not responsible for any damage due to any misunderstanding of the information in this manual. The tecan spark m10 multimode plate reader has the following modules. Tecan s commitment to cell biology research goes beyond the detection capabilities of spark 10m and, including the revolutionary fluent laboratory automation solution. Tecan spark university of massachusetts medical school. Users have access to the freedom evo operating manual, and freedom evoware software manuals, and other applicable tecan documentation, and the fragment library preparation. This freely configurable system gives researchers access to new techniques and features intended to enhance and streamline biochemical and cellbased workflows. Designed to optimize plate handling for cellbased assays, fluent automates everything from pipetting and reagent distribution to incubation and detection. User friendly spark control software allows users to easily set up and save a custom script. The spark is equipped with a 4x magnification camera and co2o2 control for live cell measurements under normoxic and hypoxic conditions.
Tecan microplate reader and cell counting spark dksh. Tecan s spark multimode microplate reader was used to identify metabolic changes during the differentiation of human mesenchymal stem cells and to assess and quantify cell viability, cytotoxicity and apoptosis, using several wellestablished and reliable cellbased assays developed by promocell. Lab manager, administrator, or technician someone who is responsible for. Increase your productivity for advanced research and drug discovery with spark, the first multimode microplate reader with integrated cooling. This is available to authorized user groups and agencies for delivery to conus, alaska, hawaii and puerto rico. It is the policy of tecan austria gmbh to improve products as new techniques and components become available. More experimental control designed to handle a broad range of common cytometry applications, spark cyto gives you a new level of experimental control without compromising on ease of use and convenience. Tecans spark multimode microplate reader was used to identify metabolic changes during the differentiation of human mesenchymal stem cells and to assess and quantify cell viability, cytotoxicity and apoptosis, using several wellestablished and reliable cellbased assays developed by promocell. Multimode microplate reader, live cell assays tecan. Fluent for research laboratories readers and washers tecan offers a range of multimode readers and washers including the infinite and spark series readers and the hydroflex and hydrospeed washers designed specifically with complete automation in mind. Bmg labtech biotek molecular devices tecan mars version 3.
From microbiology research and cellbased assays to rapid dna quantification, it delivers a combination of advanced capabilities and exceptional easeofuse to simplify your daily work. Htrf assay setup for tecan spark 1020 m multimode readers. Sorted mouse keratinocyte stem cells at karolinska institute. Tecan spark cyto the first live cell plate reader with realtime imaging and cytometry by tecan. Our systems and components help people working in clinical diagnostics, basic and translational research and drug discovery bring their science to life. Tecan s engineers and scientists are committed to delivering the highest standards of quality by focusing on the details, ensuring that your data is true and consistent, empowering your discovery. Spark multimode microplate reader from tecan selectscience.
It is also compatible with cuvettes, tecan s low volume nanoquant plate, disposable cell chips for fast cell counting in cell suspensions, and roboflask cell culture vessels for use in automated cell maintenance and assay systems. Tecan nanoquant plate quick manual pdf download manualslib. So far, i was using 100ml erlenmeyer flasks with 20 ml bacterial culture w shaking 180 rpm in 37c. Using spark tecool module for optimization of temperature.
Tecan s multimode readers provide cuvette ports for dna and rna measurement quartz or uvtransparent cuvettes must be used. Hepatitis b virus hbv causes hepatitis b, which is an infectious disease. These exercises let you launch a small ec2 cluster, load a dataset, and query it with spark, shark, spark. You have full control of the environmental conditions during a run, including the temperature and.
Jul 19, 2016 please use one of the following formats to cite this article in your essay, paper or report. The spark multimode microplate reader platform offers. It makes life much easier to researchers to track all the elisa results for further audits and commercial approvals. How to export and import scripts for tecan evoware. Multiple types of plate and wells absorbance reading with monochromator optics 200nm fluorescence top bottom reading with monochromator for exc 230900nm and em 280900nm, also stepwise intensity scans over range fluorescence polarization reading 390nm.
Using spark tecool module for optimization of temperaturesensitive. As the spark software has limitations for file handling, the powerful magellan software will be utilized to make or load and save methods, to perform measurements and to save data and worksheets workspace. Data from the pressure sensor can also be streamed in realtime to enable other pressure monitoring tasks via a host computer, such as blockage or bubble detection. Tecan microplate competitor c microplate tecan average 1. Related topics job role responsibilities integrator someone who writes software and configures hardware controlled by device drivers. The end user or routine user is a person who has suitable technical training and corresponding skills and experiences. Tecan ls series operating manual pdf download manualslib. Provides audit trail, electronic signatures, electronic records and user administration. Observe all warning and caution statements in the manual. Instructions for use reference guide ilab solutions cdn. Failure to read, understand, and follow the instructions in the manual may result in damage to the product, injury to operating personnel or poor instrument performance. Getting started learning tutorials, virtual classrooms, example files and nine different gui languages are available. Tecan spark cyto community, manuals and specifications.
Convenient automation adds full data analysis capacity to tecans automated freedom evo and fluent processing systems. Tecan spark multimode microplate reader offers everything you need to develop and run advanced biochemical and cellbased fluorescence assays. Tecan austria gmbh therefore reserves the right to change specifications at any time with. You no longer have to choose between sensitivity or flexibility you can now have both. Spark cyto can be extended with an automated multiplate cell incubator or integrated in tecan s fully automated workflow solutions. The first offices were located in a renovated farmhouse, the family home of ceo and founder, heinz abplanalp. Consumption assay user guide and phxtra glycolysis assay user guide. With the live viewer in sparkcontrol, you can document the last view before starting the experiment with the snapshot function. The tecan spark m10 multimode plate reader has the following. The gain and rfu parameters in fluorescence polarization. Oxygen consumption assay user guide and phxtra glycolysis assay. All method, data, worksheets have to be stored exclusively on the d drive. Standard operating procedures for tecan spark 10m multimode microplate reader choose the specific type of plate you are. Spark stack takes care of typical process steps, such as loading, unloading and restacking of nonlidded 6 to 1,536well plates, and provides a throughput of up to.
Tecan instructions for use for infinite 200 document part no 30017581 200807 document revision no 1. Tecan instructions for use for infinite m pro document part no. Jun 14, 2017 tecan s advanced hydrospeed plate washer. Its slender design allows the cavro adp to be mounted in automated applications with narrow 18 mm spacing between devices. The cavro adp uses air displacement to aspirate and dispense fluids. Molecular devices bmg labtech biotek tecan spectramax i3x, i3 spectramax paradigm spectramax m series. Spark cyto never miss a critical biological event with rec. You have full control of the environmental conditions during a run, including the temperature and the co 2 and o 2 levels inside the reader. The spark multimode microplate reader can effortlessly handle your routine or complex assay needs. Setting recommendations using the tecan safire2tm for the fi assays quad4 monochromators technology autofocus the autofocus feature further optimizes results by automatically adjusting the optics to the assay volume, microplate height or well shape e. Tecans spark 20m multimode microplate reader european. The contents of this manual are the property of tecan austria gmbh and are not to be copied, reproduced or transferred to another person or persons without our. These let you install spark on your laptop and learn basic concepts, spark sql, spark streaming, graphx and mllib.
The driving force behind every freedom evo application is freedom evoware, which lets you implement methods with an innovative, draganddrop graphical user interface gui. The main advantage for me is the option to buy a gmpaccomplished version. Tecan has launched the spark 20m multimode microplate reader, offering tailormade solutions to suit virtually any drug discovery or advanced life science research application. I think tecan spark 10m and magellan are very user friendly in terms of software. Fluent for research laboratories tecan pdf catalogs. Tecan reader device driver user guide 2 who should read this guide job roles this user guide is for people with the following job roles. These exercises let you launch a small ec2 cluster, load a dataset, and query it with spark, shark, spark streaming, and mllib. Page 45 autofocus area is centered automatically to the scan area. Images and confluence can be measured automatically. Instructions for use reference guide spark part no. Difference in background confluence artifacts between a tecan cell culture plate and a competitor plate using the spark reader. The cavro adp features builtin pressurebased liquid level detection plld with user configurable settings.
Spark 10m delivers unmatched performance by being the first instrument to offer independent, user selectable filters and monochromators on both the excitation and emission side for any measurement. In tecan ls with integrated barcode reader, it is also possible to set the barcode area. Tecan application notes automated solution for monitoring growth of staphylococcus aureus explore the world in tiny drops cell proliferation and cell viability analysis in in vitro systemscell culture methods on tecans infinite 200 nanoquant plate low volume dna quantification for affymetrix genechip pion assay development of pampa model for skin penetration of drugs rna quantification. Tecan was founded in 1980 by four engineers in the swiss village of hombrechtikon, with the aim to develop measurement, analysis and laboratory automation devices. New icontrol applications for dnarna quantification and measuring the labeling efficiency. Tecan is a leading global provider of automated laboratory instruments and solutions. We reserve the right to revise and change the description of this product, software and the user manual without obligation to notify users of such. Alternatively, tecan s patented nanoquant plate figure 2 is ideal for smaller volumes 2. How to properly export a scripts processesmethods for tecan evoware nowif you originally didnt correctly export your script from another system, youll need to go back to the system that contained the original script and create an export file on that system that contains all the associated definitions for that script or process.
This video will give you the basics on setting up your multimode plate reader to run cisbio htrf assays. Commercial and nonus entities are not authorized to purchase from gsa msa contracts and. Users have read important safety information related to the use of the freedom evo 150. The spark 10m multimode microplate reader is designed to offer greater flexibility and increased productivity for cell biology and genomics customers.
Using hydrospeed plate washer and infinite f50 absorbance. Designed to optimize plate handling for cellbased assays, fluent automates everything from pipetting and reagent distribution to. This freely configurable system gives researchers access to new techniques and features designed to enhance and streamline biochemical and cellbased workflows. About the quick guide every effort has been made to avoid errors in text and diagrams.
Tecan spark cyto spark cyto is a multimode plate reader with fluorescence imaging and cytometry capabilities. Tecan schweiz ag regularly offers operator training courses. The user is asked to set the scan area, the autofocus area and the agc scan area. This is complemented by builtin sample tracking, audit trails and user access controls to ensure process integrity. Tecool module allows the user to set the reader temperature. So you dont have to choose between sensitivity or flexibility for the first time ever you can have both. Fluostar optima polarstar optima cytation 1, 3, or 5 synergy h1, h4, synergy neo or neo2 spark 10m20m infinite f plex infinite f nano infinite f200pro infinite. Tecan spark allows you to check these parameters automatically with its bright field imaging and label free, laser based autofocus.
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